Pharmacognostic Characterization And Multi-Target Pharmacological Evaluation Of Carissa Carandas Leaves

Abstract

The present study investigates the pharmacognostic, phytochemical, and pharmacological potential of Carissa carandas leaves. The research involves the collection, authentication, and systematic evaluation of the plant material. Macroscopic and microscopic characteristics were studied to ensure proper identification, while physicochemical parameters such as total ash value, extractive values, moisture content, and pH determination were analyzed. Thin Layer Chromatography (TLC) was employed for fingerprint profiling, and fluorescence analysis was conducted to assess chemical characteristics.

Preliminary phytochemical screening was performed to detect major secondary metabolites qualitatively and quantitatively. Total phenolic and flavonoid content was estimated, as these compounds contribute to antioxidant activity. The antioxidant potential of the extracts was assessed using the DPPH radical scavenging method. Pharmacological evaluation included in-vivo anti-inflammatory activity using carrageenan-induced paw edema and cotton pellet-induced granuloma models.

The study aims to isolate and characterize bioactive compounds from Carissa carandas leaves, correlate their phytochemical composition with pharmacological activities, and contribute to scientific understanding and therapeutic applications. The findings may support the potential use of Carissa carandas in developing novel antioxidant and anti-inflammatory agents.