Comparative Studies Of Phytochemicals Of Acacia tortilis Of Industrial And Non-Industrial Area Of Palanpur Taluka, Banaskantha, Gujarat

Abstract

The HPLC analysis and quantification of Quercetin in methanolic extracts of Acacia tortilis reveal the presence of this bioactive compound in significant concentrations. Utilizing Quercetin as a standard, the chromatographic results exhibit a distinct retention time (Rt) of 4.1 minutes and a peak area of 41540169, indicative of its abundance in Acacia species. A standard curve established for Quercetin demonstrates excellent linearity within the range of 200-1000 mg/spot, with a high correlation coefficient (r-value) of 0.9924, ensuring precise quantification. Acacia tortilis emerges as a notable reservoir of Quercetin, with the highest concentration detected in its leaf extract at 1045.8 µg/ml, followed by the pod extract at 494.41 µg/ml. Comparative analysis with Leptadenia reticulata validates the specificity of the HPLC method, emphasizing consistency in Quercetin retention times across different plant extracts. In addition to Quercetin, the presence of other flavonoids, including p-Coumaric acid and Rutin, is quantified, enriching the flavonoid profile of Acacia tortilis. Reference to previous studies underscores the broader relevance of these findings within phytochemistry.